Coding

Part:BBa_K5207006

Designed by: Ruyi Shi   Group: iGEM24_Shanghai-BioX   (2024-09-30)


CYP76AK6

It belongs to the CYP76AK subfamily of cytochrome P450 oxygenases (oxygenases). CYP76AKs catalyze the oxidation of the C20 position.CYP76AK6 catalyzes three consecutive C20 oxidations, converting 11-hydroxyferrocenol to carnosic acid, which in turn spontaneously oxidizes to carnosol. We inserted it into pYTK001 by method above.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Figure 1. Validation plot of target gene gel electrophoresis

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